Flow-Injection Determination of L-Histidine with an Immobilized Histidine Oxidase from Brevibacillus borstelensis KAIT-B-022 and Chemiluminescence Detection
|
Accession number;06A0028771
|
| Title;Flow-Injection Determination of L-Histidine with an Immobilized Histidine Oxidase from Brevibacillus borstelensis KAIT-B-022 and Chemiluminescence Detection |
| Author;
KIBA NOBUTOSHI
(Univ. Yamanashi, Kofu, Jpn)
KOGA AYUMI
(Univ. Yamanashi, Kofu, Jpn)
TACHIBANA MASAKI
(Univ. Yamanashi, Kofu, Jpn)
TANI KAZUE
(Univ. Yamanashi, Kofu, Jpn)
KOIZUMI HITOSHI
(Univ. Yamanashi, Kofu, Jpn)
KOYAMA TETSUO
(Kanagawa Inst. Technol., Atsugi, Jpn)
YAMAMURA AKIRA
(Kanagawa Inst. Technol., Atsugi, Jpn)
MATSUMOTO KUNIO
(Kanagawa Inst. Technol., Atsugi, Jpn)
OKUDA TOHRU
(Univ. Yamanashi, Kofu, Jpn)
YOKOTSUKA KOKI
(Univ. Yamanashi, Kofu, Jpn)
|
Journal Title;Anal Sci
|
Journal Code:G0673B
|
ISSN:0910-6340
|
|
VOL.22;NO.1;PAGE.95-98(2006)
|
| Figure&Table&Reference;FIG.3, TBL.1, REF.11 |
| Pub. Country;Japan |
| Language;English |
| Abstract;A chemiluminometric flow injection analytical system for the quantitation of L-histidine is described. Histidine oxidase (EC 1.4.3.-) from Brevibacillus borstelensis KAIT-B-022 was immobilized on tresylated poly(vinyl alcohol) beads and packed into a stainless-steel column. The hydrogen peroxide produced was detected chemiluminometrically by a flow-through sensor containing immobilized peroxidase (EC 1.11.1.7). The maximum sample throughput was 10 h'-1'. The calibration graph was linear from 0.05 to 5 mM; the detection limit (signal to noise ratio = 3) was 0.01 mM. The activity of immobilized histidine oxidase reduced to 65% of the initial value after 350 injections. The system was applied to the determination of L-histidine in fish meat, such as salmon, tunny, bonito, and mackerel. (author abst.) |
|
|
|
Related Articles;
|
|
|
