Ratiometric Fluorescence Detection of Pyrimidine/Purine Transversion by Using a 2-Amino-1,8-naphthyridine Derivative
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Accession number;06A0104543
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| Title;Ratiometric Fluorescence Detection of Pyrimidine/Purine Transversion by Using a 2-Amino-1,8-naphthyridine Derivative |
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Journal Title;Anal Sci
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Journal Code:G0673B
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ISSN:0910-6340
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VOL.22;NO.2;PAGE.195-197(2006)
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| Figure&Table&Reference;FIG.3, REF.19 |
| Pub. Country;Japan |
| Language;English |
| Abstract;A new class of abasic site-binding fluorescence ligands, Naph-NBD in which 7-nitrobenzo-2-oxa-1,3-diazole (NBD) is connected to 2-amino-7-methyl-1,8-naphthyridine (Naph) by a propylene linker, is presented for the ratiometric assay for SNPs typing. In solutions buffered to pH 7.0 (I = 0.11 M, at 5.DEG.C.), Naph-NBD is found to selectively recognize pyrimidine bases over purine bases opposite the abasic site in DNA duplexes (K11/M'-1': T, 8.1 * 10'6'; C, 2.5 * 10'6'; G, 0.33 * 10'6'; A, 0.27 * 10'6'). The binding of Naph-NBD is accompanied by significant quenching of the fluorescence from the naphthyridine moiety (.LAMBDA.max, 409 nm), while the emission from the NBD (.LAMBDA.max, 544 nm) is relatively unaffected. Such a fluorescence response of Naph-NBD allows the emission ratio detection of pyrimidine/purine transversion. (author abst.) |
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