Science Links Japan | Enhancement of the Binding Ability of a Ligand for Nucleobase Recognition by Introducing a Methyl Group

Enhancement of the Binding Ability of a Ligand for Nucleobase Recognition by Introducing a Methyl Group

Accession number;06A0104545

Title;Enhancement of the Binding Ability of a Ligand for Nucleobase Recognition by Introducing a Methyl Group

Author;

Journal Title;Anal Sci

Journal Code:G0673B

ISSN:0910-6340

VOL.22;NO.2;PAGE.201-203(2006)

Figure&Table&Reference;FIG.3, TBL.1, REF.19

Pub. Country;Japan

Language;English

Abstract;The recognition ability of pteridine derivatives for nucleobases opposite an abasic (AP) site in an oligodeoxynucleotide (ODN) duplex is enhanced by using a propylene residue (Spacer-C3) as an AP site. The recognition ability is further enhanced both by attaching methyl groups to a fluorescent ligand and by measuring the fluorescence response at 5.DEG.C.; 6.2 * 10'6' M'-1' of the binding constant is attained between 2-amino-6,7-dimethyl-4-hydroxypteridine and guanine opposite the AP site in water. (author abst.)

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