UV Light-Emitting Diode-Induced Fluorescence Detection Combined with Online Sample Concentration Techniques for Capillary Electrophoresis
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Accession number;06A0104551
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| Title;UV Light-Emitting Diode-Induced Fluorescence Detection Combined with Online Sample Concentration Techniques for Capillary Electrophoresis |
| Author;
CHANG YEN-SHENG
(National Taiwan Normal Univ., Taipei, Twn)
SHIH CHUN-MIN
(National Taiwan Normal Univ., Taipei, Twn)
LIN CHENG-HUANG
(National Taiwan Normal Univ., Taipei, Twn)
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Journal Title;Anal Sci
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Journal Code:G0673B
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ISSN:0910-6340
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VOL.22;NO.2;PAGE.235-240(2006)
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| Figure&Table&Reference;FIG.6, TBL.1, REF.32 |
| Pub. Country;Japan |
| Language;English |
| Abstract;The application of an ultraviolet (UV) light-emitting diode (LED) to on-line sample concentration/fluorescence detection in capillary electrophoresis (CE) is described. The utility of a UV-LED (peak emission wavelength at 380 nm, -2 mW) for fluorescence detection was demonstrated by examining both a naturally fluorescent (riboflavin) compound and a non-fluorescent compound (tryptophan), respectively. The detection limit for riboflavin was determined to be 0.2 ppm by the normal MEKC mode, which was improved to 3 - 7 ppb when dynamic pH-junction technique was applied. On the other hand, the detection limit of the tryptophan derivative was determined to be 1.5 ppm using the MEKC mode, which was improved to 3 ppb when the sweeping-MEKC mode was applied. In an analysis of an actual sample, the concentrations of riboflavin in beer, and tryptophan in urine and milk samples were determined, respectively. (author abst.) |
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