The Degree of Degraded Proteoglycan in Human Vitreous and the Influence of Peroxidation
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Accession number;06A0171394
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| Title;The Degree of Degraded Proteoglycan in Human Vitreous and the Influence of Peroxidation |
| Author;
TAKAHASHI KAZUNORI
(Koshigaya Hospital, Dokkyo Univ. School of Medicine, JPN)
ARAI KIYOMI
(Koshigaya Hospital, Dokkyo Univ. School of Medicine, JPN)
HAYASHI SHINMIN
(Koshigaya Hospital, Dokkyo Univ. School of Medicine, JPN)
TANAKA YASUSHI
(Koshigaya Hospital, Dokkyo Univ. School of Medicine, JPN)
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Journal Title;Journal of Japanese Ophthalmological Society
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Journal Code:Z0666A
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ISSN:0029-0203
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VOL.110;NO.3;PAGE.171-179(2006)
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| Figure&Table&Reference;FIG.8, TBL.2, REF.25 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Purpose: The degradation of hyaluronic acid induced by reactive oxygen species has been reported in vitro as a cause of vitreous liquefaction. In this study on in vivo human vitreous liquefaction, we examined the degree of degraded proteoglycan (PG) in human vitreous and the influence of peroxidation. Subjects and Methods: In this study we examined the vitreous of 5 eyes with diabetic retinopathy, 12 eyes with retinal detachment, 2 eyes with retinal vein occlusion, and one eye with macular hole. The degree of degraded PG was determined by cellulose acetate membrane electrophoresis. To evaluate the influence of oxidation, copper ions, iron ions, hydrogen peroxide, lipid peroxide, L-ascorbic acid concentrations, and superoxide scavenging activity were measured. Results: The electrophoretic patterns of PG were classified into the origin type (non-fragmented PG) in which only the origin was stained, and the mobile type (fragmented PG) showed other patterns. In the mobile type, the copper ion and hydrogen peroxide concentrations were higher, and the lipid peroxide concentrations were significantly higher, while the superoxide scavenging activity was lower. Conclusion: These results indicated that the degradation of PG and dissociation of glycosaminoglycan in human vitreous were associated with peroxidation. (author abst.) |
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