Genetic Transformation of 'Kyoho' Grape with a GFP Gene
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Accession number;06A0224897
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| Title;Genetic Transformation of 'Kyoho' Grape with a GFP Gene |
| Author;
NAKAJIMA IKUKO
(National Inst. Of Fruit Tree Sci., Tsukuba, Jpn)
MATSUTA NAGAO
(National Inst. Of Fruit Tree Sci., Tsukuba, Jpn)
YAMAMOTO TOSHIYA
(National Inst. Of Fruit Tree Sci., Tsukuba, Jpn)
TERAKAMI SHINGO
(Univ. Tsukuba, Tsukuba, Jpn)
SOEJIMA JUNICHI
(National Inst. Of Fruit Tree Sci., Tsukuba, Jpn)
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Journal Title;Journal of the Japanese Society for Horticultural Science
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Journal Code:F0626A
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ISSN:0013-7626
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VOL.75;NO.2;PAGE.188-190(2006)
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| Figure&Table&Reference;FIG.2, TBL.1, REF.8 |
| Pub. Country;Japan |
| Language;English |
| Abstract;'Kyoho' grapevine (Vitis * labruscana Bailey) was successfully transformed by Agrobacterium-mediated transformation. Embryogenic calli (EC) were co-cultured with A. tumefaciens strain, EHA105 that harbors a green fluorescent protein (GFP) gene with the CaMV35S promoter. Kanamycin at 15 mg L'-1' was suitable for isolating transformed EC from which three plants that express GFP fluorescence were regenerated. DNA analyses, including polymerase chain reaction (PCR) and PCR-Southern hybridization, confirm that these three plants contain sequences comparable to the GFP gene to indicate that it was successfully incorporated into the 'Kyoho' grapevine genome. (author abst.) |
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