Chylomicron Remnants Stimulate Release of Interleukin-1.BETA. by THP-1 Cells
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Accession number;06A0174933
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| Title;Chylomicron Remnants Stimulate Release of Interleukin-1.BETA. by THP-1 Cells |
| Author;
OKUMURA TAKAHIRO
(Dep. Of Internal Medicine, Div. Of Coronary Heart Disease, Hyogo Coll. Of Medicine, Nishinomiya, Jpn)
FUJIOKA YOSHIO
(Dep. Of Internal Medicine, Div. Of Coronary Heart Disease, Hyogo Coll. Of Medicine, Nishinomiya, Jpn)
FUJIOKA YOSHIO
(Present Address; Div. Of Cardiovascular And Respiratory Medicine, Dep. Of Internal Medicine, Kobe Univ. Of Graduate ...)
MORIMOTO SHINJI
(Dep. Of Internal Medicine, Cardiovascular Div., Hyogo Coll. Of Medicine, Nishinomiya, Jpn)
MASAI MIHO
(Dep. Of Internal Medicine, Div. Of Coronary Heart Disease, Hyogo Coll. Of Medicine, Nishinomiya, Jpn)
SAKODA TSUYOSHI
(Dep. Of Internal Medicine, Div. Of Coronary Heart Disease, Hyogo Coll. Of Medicine, Nishinomiya, Jpn)
TSUJINO TAKESHI
(Dep. Of Internal Medicine, Cardiovascular Div., Hyogo Coll. Of Medicine, Nishinomiya, Jpn)
KASHIWAMURA SHIN-ICHIRO
(Lab. Of Host Defenses, Inst. For Advanced Medical Sciences, Hyogo Coll. Of Medicine, Nishinomiya, Jpn)
OKAMURA HARUKI
(Lab. Of Host Defenses, Inst. For Advanced Medical Sciences, Hyogo Coll. Of Medicine, Nishinomiya, Jpn)
OHYANAGI MITSUMASA
(Dep. Of Internal Medicine, Div. Of Coronary Heart Disease, Hyogo Coll. Of Medicine, Nishinomiya, Jpn)
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Journal Title;J Atheroscler Thromb
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Journal Code:L2187A
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ISSN:1340-3478
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VOL.13;NO.1;PAGE.38-45 (J-STAGE)(2006)
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| Figure&Table&Reference;FIG.5, REF.41 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Recent findings suggest that the oxidative modification of low-density lipoproteins (LDL) and an increase in triglyceride-rich lipoprotein particles including chylomicron remnants contribute to the progression of atherosclerosis, as does the inflammatory response. We therefore examined whether and how these lipoproteins affected interleukin (IL)-1.BETA. release and mRNA expression for IL-1.BETA. and IL-18 in THP-1 cells, a human monocyte cell line. Chylomicron remnants increased IL-1.BETA. release into the conditioned medium by THP-1 in a dose- and time-dependent manner. At concentrations up to 1 .MU.g/ml, chylomicron remnants increased IL-1.BETA. release by 4-fold compared with the control. Neither native LDL nor oxidized LDL (OxLDL) significantly increased IL-1.BETA. release. Chylomicron remnants increased IL-1.BETA. mRNA expression by 3 times. Native LDL or OxLDL did not increase IL-1.BETA. mRNA, while neither these lipoproteins nor chylomicron remnants increased IL-18 mRNA. Chylomicron remnants also increased the activities of caspase-1 and nuclear factor (NF)-.KAPPA.B significantly, while native LDL or OxLDL did not. In conclusion, chylomicron remnants stimulated IL-1.BETA. mRNA expression and IL-1.BETA. protein production probably via caspase-1 and NF-.KAPPA.B activation in THP-1 cells. (Author abst.) |
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