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Accession number;06A0294330
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| Title;Detection of Bartonella DNA in Cat Blood with Nested-PCR |
| Author;
TOMITA MASAAKI
(Yamaguchi Prefectural Res. Inst. Health, JPN)
KUDO EMI
(Yamaguchi Prefectural Res. Inst. Health, JPN)
TOMINAGA KIYOSHI
(Yamaguchi Prefectural Res. Inst. Health, JPN)
MATSUMURA KENDO
(Yamaguchi Prefectural Res. Inst. Health, JPN)
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Journal Title;Journal of the Japan Veterinary Medical Association
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Journal Code:Y0220A
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ISSN:0446-6454
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VOL.59;NO.4;PAGE.275-278(2006)
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| Figure&Table&Reference;TBL.2, REF.13 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Three different nested-PCR assays were evaluated in terms of their ability to detect Bartonella DNA in cat-blood samples. The primer pairs used were parts of the citrate synthase (gltA) gene, heat shock protein (htrA) gene and 16S-23S rRNA intergenic region (ITS) gene, respectively. Of the 29 culture-positive cats-blood samples, Bartonella DNA was detected in 20 (68.9%) by the gltA nested-PCR and htrA nested-PCR. The ITS nested-PCR detected Bartonella DNA in 26 of 29 (89.7%). In two of 29 specimens, none of the gene examined was detected. These results indicate that the nested-PCR is a useful tool for the detection of Bartonella DNA in cat-blood samples. (author abst.) |
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