Introduction of Odontoglossum ringspot virus Coat Protein Gene into Cymbidium niveo-marginatum mediated by Agrobacterium tumefaciens to Produce Transgenic Plants
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Accession number;06A0413496
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| Title;Introduction of Odontoglossum ringspot virus Coat Protein Gene into Cymbidium niveo-marginatum mediated by Agrobacterium tumefaciens to Produce Transgenic Plants |
| Author;
CHEN LI
(Hiroshima Prefectural Univ., Shobara, Jpn)
KAWAI HITOMI
(Hiroshima Prefectural Univ., Shobara, Jpn)
OKU TAKASHI
(Hiroshima Prefectural Univ., Shobara, Jpn)
TAKAHASHI CHISAKO
(Mukaijima Orchid Center, Hiroshima, Jpn)
NIIMI YOSHIYUKI
(Hiroshima Prefectural Univ., Shobara, Jpn)
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Journal Title;Journal of the Japanese Society for Horticultural Science
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Journal Code:F0626A
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ISSN:0013-7626
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VOL.75;NO.3;PAGE.249-255(2006)
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| Figure&Table&Reference;FIG.5, REF.39 |
| Pub. Country;Japan |
| Language;English |
| Abstract;We cloned the Odontoglossum ringspot virus (ORSV) coat protein (CP) gene from ORSV-infected Epidendrum and constructed a pHPUNOJE-1 vector by ligating this CP gene, green fluorescence protein (GFP) gene, and hygromycin resistant (Hyg'r') gene into the plasmid pGreenII. This chimeric vector was mediated into A. tumefaciens LBA 4404. By infecting and co-culturing the rhizomes of Cym. niveo-marginatum with A. tumefaciens LBA 4404, ORSV CP gene transgenic shoots were obtained. GFP was observed in 64% of the 81 surviving rhizomes and in 39% of 215 regenerated shoots. The presence of ORSV CP, GFP, and Hyg'r' genes was confirmed in the transgenic shoots by using polymerase chain reaction (PCR) analysis, whereas that of ORSV CP was confirmed by enzyme-linked immunosorbent assay (ELISA). (author abst.) |
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