Rapid Screening Assay for Ripe Rot Resistance in Grape Cultivars
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Accession number;06A0413498
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| Title;Rapid Screening Assay for Ripe Rot Resistance in Grape Cultivars |
| Author;
SHIRAISHI MIKIO
(National Inst. Of Fruit Tree Sci., Higashihiroshima, Jpn)
YAMADA MASAHIKO
(National Inst. Of Fruit Tree Sci., Higashihiroshima, Jpn)
MITANI NOBUHITO
(National Inst. Of Fruit Tree Sci., Higashihiroshima, Jpn)
UENO TOSHIHITO
(National Inst. Of Fruit Tree Sci., Higashihiroshima, Jpn)
NAKAUNE RYOJI
(National Inst. Of Fruit Tree Sci., Higashihiroshima, Jpn)
NAKANO MASAAKI
(National Inst. Of Fruit Tree Sci., Higashihiroshima, Jpn)
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Journal Title;Journal of the Japanese Society for Horticultural Science
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Journal Code:F0626A
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ISSN:0013-7626
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VOL.75;NO.3;PAGE.264-266(2006)
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| Figure&Table&Reference;FIG.3, REF.6 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Ripe rot caused by Colletotrichum acutatum (CA) has been the most serious preharvest disease of grape berries/clusters. Furthermore, the pathogen is highly resistant to benomyl and iminoctadine-triacetate. A rapid assay for screening ripe rot (CA) resistance of breeding materials in table grapes was developed in which the harvested berries are washed with water and surface-sterilized in ethanol. The berries were then inoculated with conidial suspensions (ca. 3.0*10'5' spores/mL per ten berries) in a polyethylene bag, and incubated at 25.DEG.C. in the dark for 2 weeks. The percentage of diseased berries increased as the ripening of berries proceeded. A period of 70 to 80 days after full bloom was suitable for berry sampling and subsequent CA inoculation to evaluate varietal differences in the ripe rot resistance of grapevine. (author abst.) |
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