Pulmonary Swab Sampling Using Trocar from Pigs Dying from Pneumonia

Accession number;06A0478030
Title;Pulmonary Swab Sampling Using Trocar from Pigs Dying from Pneumonia
Author; ARIKAWA AKINOBU (Miyazaki Univ., Faculty of Agriculture, JPN) ARIKAWA AKINOBU (Miyazaki Prefect. Fed. Agric. Mutual Relief Assoc., JPN) SUEYOSHI MASUO (Miyazaki Univ., Faculty of Agriculture, JPN) NAGATOMO HIROSHI (Miyazaki Univ., Faculty of Agriculture, JPN) TAURA YASUHO (Yamaguchi Univ., Fac. Agriculture, JPN)
Journal Title;Journal of the Japan Veterinary Medical Association
Journal Code:Y0220A
ISSN:0446-6454
VOL.59;NO.6;PAGE.385-388(2006)
Figure&Table&Reference;FIG.4, TBL.1, REF.11
Pub. Country;Japan
Language;Japanese
Abstract;We devised a safe and simple method to obtain pulmonary specimens for bacterial examination from pigs that died from pneumonia. Nidal lung tissues, pulmonary swabs, nasal cavity swabs and tonsils were obtained from 30 dead pigs with respiratory symptoms on 15 farms. The pulmonary swab specimens were obtained by using the trocar for cows. The trocar was stuck into the chest of dead pigs and the swab that was inserted after the inner core was removed was obtained for the pulmonary specimen. The rate of isolation of Actinobacillus pleuropneumoniae from nidal lung tissues, pulmonary swabs, tonsils and nasal cavity swabs in 26 pigs suspected of pleuropneumonia by macroscopic findings were 92% (24/26), 81% (21/26), 59% (10/17) and 31% (8/26), respectively. Since we could obtain the pulmonary materials from the dead pigs using the trocar, an autopsy of the pigs were not mandatory for the sampling of bacterial specimen from the affected lungs, and thus the propagation of the pathogen in the farm could be avoided. It was suggested that the present method was useful. (author abst.)