Detection and Genotyping of Flavobacterium psychrophilum by PCR targeted to Peptidyl-prolyl cis-trans isomerase C Gene
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Accession number;06A0575252
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| Title;Detection and Genotyping of Flavobacterium psychrophilum by PCR targeted to Peptidyl-prolyl cis-trans isomerase C Gene |
| Author;
YOSHIURA YASUTOSHI
(Fisheries Res. Agency, Mie, Jpn)
KAMAISHI TAKASHI
(Fisheries Res. Agency, Mie, Jpn)
NAKAYASU CHIHAYA
(Fisheries Res. Agency, Mie, Jpn)
OTOTAKE MITSURU
(Fisheries Res. Agency, Mie, Jpn)
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Journal Title;Fish Pathology
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Journal Code:G0015B
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ISSN:0388-788X
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VOL.41;NO.2;PAGE.67-71(2006)
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| Figure&Table&Reference;FIG.3, TBL.1, REF.5 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Flavobacterium psychrophilum can be classified into two genotypes, A and B, by the polymorphism in an unexpected product of 290bp that is amplified with universal primers for gyrase subunit B gene. We identified the 290bp fragment as a part of coding region of peptidyl-prolyl cistrans isomerase C (PPIC) gene. Using PCR newly designed for PPIC gene, specific amplification was observed in all F.psychrophilum strains tested, and no amplification occurred in any other bacterial species tested. Moreover, genotype A was found only in isolates from ayu Plecoglossus altivelis. (author abst.) |
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