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Accession number;06A0586487
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| Title;Construction of ELISA System to Detect NPT II Protein in Genetically Modified Foods |
| Author;
SASAKI KAZUO
(Aomori Univ.)
UMETSU HIRONORI
(Aomori Univ.)
YAMADA AKIYO
(Tokyo Univ. Agriculture And Technol.)
KAMADA HIROSHI
(Univ. Tsukuba)
OZEKI YOSHIHIRO
(Tokyo Univ. Agriculture And Technol.)
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Journal Title;Japanese Journal of Food Chemistry
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Journal Code:L4470A
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ISSN:1341-2094
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VOL.12;NO.3;PAGE.140-144(2005)
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| Figure&Table&Reference;FIG.5, REF.3 |
| Pub. Country;Japan |
| Language;English |
| Abstract;An ELISA detection system specific for neomycin phosphotransferase II (NPT II) protein was constructed using the antibodies against overexpressed NPT II protein in recombinant Escherichia coli. NPT II protein in genetically modified (GM) tobacco plants could be qualitatively detected using this system; however, non-specific detection in normal tobacco, soybean, corn and potato was not observed. These results suggest that the constructed ELISA system can monitor NPT II protein in GM foods. (author abst.) |
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