Detection and quantification of aldehyde dehydrogeanase activities in Japanese young female students using genetic and psychiatrist tests of the alcoholism
|
Accession number;06A0586488
|
| Title;Detection and quantification of aldehyde dehydrogeanase activities in Japanese young female students using genetic and psychiatrist tests of the alcoholism |
| Author;
YANO MEGUMU
(Mukogawa Women's Univ., Fac. of Human Environmental Sci., JPN)
TOYASAKI MAMI
(Mukogawa Women's Univ., Fac. of Human Environmental Sci., JPN)
TAKII YUKIO
(Mukogawa Women's Univ., Fac. of Human Environmental Sci., JPN)
TAKII YUKIO
(Mukogawa Women's Univ., JPN)
|
Journal Title;Japanese Journal of Food Chemistry
|
Journal Code:L4470A
|
ISSN:1341-2094
|
|
VOL.12;NO.3;PAGE.145-151(2005)
|
| Figure&Table&Reference;FIG.9, TBL.4, REF.15 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Genetic and epidemiological studies on alcohol tolerance frequencies were conducted by analyzing genetic materials from 149 healthy female students. Aldehyde dehydrogenase 2 (ALDH2) gene was characterized by the combination of polymerase chain reaction and agarose gel electrophoresis of respective DNA specimen. Nail clippings were found to be relatively easy to collect and extract DNA from volunteers especially when they were restricted to female students. Plucking hair roots was not successful, since it caused sometimes pains on the volunteers. Among 149 individuals tested, 65.6% were the typical or normal homozygote, 32.2% were the heterozygote, and 2.0% were the atypical mutant homozygote. The reliability of the scale ratings using ALDH2 genotype analysis was assessed and confirmed by ethanol patch test and other psychiatrist's evaluation methods, such as TAST (Tokyo-University ALDH2-Phenotype Screening Test). However, the genotype analysis is not directly associated with AAIS (Adolescent Alcohol Involvement Scale). Accordingly, careful case-control study must be undertaken on genotype analysis and it must be accompanied by several combination of epidemiological studies. (author abst.) |
|
|
|
Related Articles;
|
|
