Metabolic behavior of enzymatically modified isoquercitrin by .ALPHA.-amylase and gastric juices

Accession number;06A0586489
Title;Metabolic behavior of enzymatically modified isoquercitrin by .ALPHA.-amylase and gastric juices
Author; TAMURA SATORU (Osaka Univ.) TSUJI KOJI (Osaka Univ.) MORIWAKI MASAMITSU (San-ei Gen F.f.i., Inc.) MURAKAMI NOBUTOSHI (Osaka Univ.)
Journal Title;Japanese Journal of Food Chemistry
Journal Code:L4470A
ISSN:1341-2094
VOL.12;NO.3;PAGE.152-156(2005)
Figure&Table&Reference;FIG.3, REF.6
Pub. Country;Japan
Language;English
Abstract;In order to elucidate metabolic behavior of enzymatically modified isoquercitrin (EMI) after oral ingestion, reactants of main eight constituents treated with salivary and pancreatic .ALPHA.-amylase, and gastric juices were respectively analyzed by HPLC quantitation. Among the eight constituents, isoquercitrin (IQC), IG1, and IG2 were revealed to be little submitted to both .ALPHA.-amylase catalyzed hydrolysis. In addition, the remaining five constituents IG3-IG7 were shown to be completely metabolized by the two .ALPHA.-amylase, taking exposure time in the body into account. On the other hand, gastric juice treatment provided no reactants with respect to all substrates even in the coexistence of salivary .ALPHA.-amylase. Consequently, EMI through oral ingestion was deduced to be converged to the three components, IQC, IG1, and IG2, by the action of salivary and pancreatic .ALPHA.-amylase prior to the small intestine. (author abst.)