Hemocyte of Clam Shrimp, Leptestheria kawachieusis (Conchostraca, Branchiopoda, Crustacea)
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Accession number;06A0769756
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| Title;Hemocyte of Clam Shrimp, Leptestheria kawachieusis (Conchostraca, Branchiopoda, Crustacea) |
| Author;
KONDO MASAKAZU
(National Fisheries Univ., JPN)
INAGAWA HIROYUKI
(National Fisheries Univ., JPN)
TOMONAGA SUSUMU
(Shoyogakuin)
TAKAHASHI YUKINORI
(National Fisheries Univ., JPN)
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Journal Title;Journal of National Fisheries University
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Journal Code:F0239A
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ISSN:0370-9361
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VOL.54;NO.4;PAGE.153-157(2006)
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| Figure&Table&Reference;FIG.3, REF.7 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Morphological and cytochemical characteristics of hemocyte in a clam shrimp, Leptestheria kawachieusis, were examined by light microscopy. Only a single type of hemocyte, granulocyte, were observed in the hemolymph. This hemocyte was small (4.0-7.0.MU.m in diameter), round or oval in shape and characterized by large granule. The granule was round or oval (1.4.MU.m in diameter) and stained eosinophilic with May-Gruenwald using distilled water (pH 5.2) or acid phosphate buffer (pH 5.0 and pH 6.0), basophilic at pH7.0 and pH8.0. Furthermore, the granules were stained with eosin, methylene blue, azure A and safranine O. These results indicate that the granule is amphophilic. Although the granule was stained with toluidine blue and sudan black B, PAS, alcian blue, oil red O and sudan III were negative in the granule. Glycogen was not detected in the hyaloplasm. Phenoloxidase activity was observed in the granule. (author abst.) |
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