Search for mutant genes in the cultured green-type mutant strain of Porphyra yezoensis

Accession number;06A0769760
Title;Search for mutant genes in the cultured green-type mutant strain of Porphyra yezoensis
Author; MIZUKAMI YUZURU (National Fisheries Univ., JPN) KAWASAKI TAKEHITO (National Fisheries Univ., JPN) MURASE NOBORU (National Fisheries Univ., JPN) KUNIMOTO MASAHIKO (National Res. Inst. Fisheries Sci., Fisheries Res. Agency, JPN)
Journal Title;Journal of National Fisheries University
Journal Code:F0239A
ISSN:0370-9361
VOL.54;NO.4;PAGE.181-188(2006)
Figure&Table&Reference;FIG.4, TBL.3, REF.20
Pub. Country;Japan
Language;Japanese
Abstract;The green-type pigmentation mutant strain of Porphyra yezoensis was examined for the genomic genes related to the pigmentation mutation. Although this strain is a pigmentation variant, the nucleotide sequences of six phycobilin-related genes and ribulose bisphosphate carboxylase-oxygenase (Rubisco) coding regions did not vary between the wild and mutant-type strains. The RAPD analysis of the total genomic DNA showed 80.1 % similarity of DNA fragments between these two strain types in the electrophoretic mobility. From DNA fragments specific for the wild or mutant strain in the RAPD pattern, eight fragments (eight loci) were chosen arbitrarily for the analysis of nucleotide sequences. Three of the fragments consisted of nucleotide sequences similar to those of retrotransposons in higher plants. For other five fragments, no corresponding gene was specified by the gene bank searches. (author abst.)