Analysis of mutant genes in cultured mutant strain of Porphyra yezoensis

Accession number;06A0769761
Title;Analysis of mutant genes in cultured mutant strain of Porphyra yezoensis
Author; MIZUKAMI YUZURU (National Fisheries Univ., JPN) KAWASAKI TAKEHITO (National Fisheries Univ., JPN) MURASE NOBORU (National Fisheries Univ., JPN) KUNIMOTO MASAHIKO (National Res. Inst. Fisheries Sci., Fisheries Res. Agency, JPN)
Journal Title;Journal of National Fisheries University
Journal Code:F0239A
ISSN:0370-9361
VOL.54;NO.4;PAGE.189-195(2006)
Figure&Table&Reference;FIG.5, TBL.1, REF.12
Pub. Country;Japan
Language;Japanese
Abstract;Nucleotide sequences in four loci of the Porphyra yezoensis genome were compared between wild and green-type pigmentation mutant strains. Approximately 3 to 10 % of nucleotides varied in two loci by substitutions, additions and deletions of nucleotides. However, in the other two loci, nucleotide sequences were identical between the two strain types although previous data of RAPD analysis suggested variations in the nucleotide sequences. Southern-blot analysis showed that all of the nucleotide sequences found in the 4 loci were repeated over the genome and the frequency of repetition differed between the two strain types. Nucleotide sequences in two loci showed 100 % nucleotide similarities with those of retrotransposons in higher plants. These results suggested that the spontaneous mutation of Porphyra yezoensis was brought about by retrotransposons as well as nucleotide conversions. (author abst.)