Is the dry weight changed by the fixation and staining at the samplinf of root system?
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Accession number;06A0798335
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| Title;Is the dry weight changed by the fixation and staining at the samplinf of root system? |
| Author;
OGAWA ATSUSHI
(Akita Prefectural Univ., JPN)
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Journal Title;Root Research
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Journal Code:L2698A
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ISSN:0919-2182
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VOL.15;NO.3;PAGE.125-128(2006)
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| Figure&Table&Reference;FIG.2, TBL.1, REF.5 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The morphological characters in root systems, such as root length, are usually measured after fixation (and staining) and it is assumed that the dry weight without fixation is different from that with fixation (and staining). In this study, the relationship between root length and dry weight was investigated for samples without fixation ("Method 1"), with fixation ("Method 3"), and with fixation and staining ("Method 2"), and the effect of fixation and staining on dry weight was examined. The correlation between root length and dry weight was assessed, and the resulting regression slopes of the three treatments were compared. There were no significant differences among the regression slopes. When the dry weight per root length resulting from the three treatments were compared, there was a significant difference between "Method 1" and "Method 2 or 3", such that the dry weight per root length of "Method 2 and 3" was about 70% that of "Method 1". This result shows that dry weight is decreased about 30% by the fixation. These results suggest that when the dry weight is measured after fixation, the dry weight can be compared relatively, and the possibility that it may be different from true dry weight must be considered. (author abst.) |
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