Science Links Japan | Swing Activation of FEN1 Revealed by the Crystal Structure of Human FEN1-PCNA Complex

Swing Activation of FEN1 Revealed by the Crystal Structure of Human FEN1-PCNA Complex

Accession number;06A0976111

Title;Swing Activation of FEN1 Revealed by the Crystal Structure of Human FEN1-PCNA Complex

Author; KITANO KEN (Advanced Inst. Sci. and Technol., Nara) HAKOSHIMA TOSHIO (Advanced Inst. Sci. and Technol., Nara)

Journal Title;Journal of the Crystallographic Society of Japan

Journal Code:G0232A

ISSN:0369-4585

VOL.48;NO.5;PAGE.367-372(2006)

Figure&Table&Reference;FIG.6, REF.13

Pub. Country;Japan

Language;Japanese

Abstract;Flap endonuclease-1(FEN1)removes the RNA primer during Okazaki fragment maturation in lagging strand DNA synthesis. FEN1 also plays a crucial role in the long-patch base excision repair. FEN1 endonuclease activity is markedly stimulated by binding to proliferating cell nuclear antigen(PCNA), which is well known as the 'DNA sliding clamp'. We determined the crystal structure of human FEN1 complexed to PCNA. A hinge region found in FEN1 endows the enzyme with a degree of freedom to swing and direct its core domain toward the flap-DNA substrate. (author abst.)

Related Articles;