The analysis on the treatment and the immunological/allergic mechanisms of atopic dermatitis. From the aspect of T cell function (Immunomodulating action by environmental pollutants)
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Accession number;06A1040528
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| Title;The analysis on the treatment and the immunological/allergic mechanisms of atopic dermatitis. From the aspect of T cell function (Immunomodulating action by environmental pollutants) |
| Author;
AIBA SETSUYA
(Tohoku Univ., Graduate School of Medicine, JPN)
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Journal Title;Skin Research
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Journal Code:Z0014C
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ISSN:1347-1813
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VOL.5;NO.Suppl.7;PAGE.5-10(2006)
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| Figure&Table&Reference;FIG.8, REF.30 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;There is growing evidence that diesel exhaust particles (DEP) or folmaldehyde (FA) can induce allergic diseases with increased IgE production and preferential activation of Th2 cells. To clarify the cellular basis of the role of DEP and FA in the induction of Th2-dominant responses, we examined the effects of DEP or FA on the cytokine production by T-cells stimulated with anti-CD3/CD28 antibody and on that by monocyte-derived dendritic cells (MoDCs) stimulated with CD40 ligand and/or IFN-.GAMMA.. We examined IFN-.GAMMA., IL-4, IL-5, IL-8, and IL-10 produced by T-cells and TNF-a, IL-1b, IL-10, and IL-12 produced by MoDCs using real-time PCR analysis or by ELISA. To highlight the effects of DEP or FA, we compared the effects of DEP with those of dexamethasone (DEX) and cyclosporine A(CyA). Both DEP and FA significantly suppressed INF-.GAMMA. mRNA expression and protein production, while it did not affect IL-4 or IL-5 mRNA expression or protein production. Both DEP and FA suppressed IL-12p40 and IL-12p35 mRNA expression and IL-12p40 production by MoDCs, while it augmented IL-1b mRNA expression. Finally, by using a thiol antioxidant, N-acetylcysteine (NAC), we found that the suppression of INF-g production by DEP- or FA-treated T cells was mediated by oxidative stress. These data revealed a unique characteristic of DEP, namely that they induce a Th2 cytokine milieu in both T cells and DCs. Next, to explore the molecular events underlying the diminished IFN-g and IL-10 production by DEP- or FA-treated T cells, we examined their effects on mRNA expression by T cells stimulated with anti-CD3/anti-CD28 mAb using microarrays and real-time PCR. By real-time PCR, we found that both DEP and FA significantly suppressed mRNA expression of T-bet, Txk and c-Maf but not that of GATA-3, SOCS3, SOCS5, or Gadd45b.... (author abst.) |
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