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Accession number;07A0049620
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| Title;Sodium chloride-enhanced adherence of Edwardsiella tarda to HEp-2 cells |
| Author;
MAHMOUD MAHMOUD MOSTAFA
(Hiroshima Univ., Higashi-hiroshima, Jpn)
OKUDA JUN
(Hiroshima Univ., Higashi-hiroshima, Jpn)
NAKAI TOSHIHIRO
(Hiroshima Univ., Higashi-hiroshima, Jpn)
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Journal Title;Fish Pathology
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Journal Code:G0015B
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ISSN:0388-788X
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VOL.41;NO.4;PAGE.165-170(2006)
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| Figure&Table&Reference;FIG.4, TBL.1, REF.29 |
| Pub. Country;Japan |
| Language;English |
| Abstract;The adherence of Edwardsiella tarda strains to HEp-2 cells was studied by the viable cell count and direct microscopic count methods. Three selected E. tarda strains having different hemagglutination activities against guinea pig erythrocytes were cultured in a peptone-yeast extract broth supplemented with 3% NaCl (3%-NaCl culture) or without NaCl (0%-NaCl culture). Strain FK1051 showed significantly higher adherence in the 3%-NaCl culture than in the 0%-NaCl culture. A similar adherence pattern was observed in strain KG8401, though the adherence of the 0%-NaCl culture was higher than that of FK1051. In contrast, both cultures of strain SU166 were low in the adherence. These adherence patterns to the HEp-2 cells correlated well with the previously reported hemagglutinating activities of the strains. N-acetylneuraminic acid markedly inhibited the adherence as well as the hemagglutination. This NaCl-enhanced adherence may be involved in pathogenesis of E. tarda infection particularly in marine fish. (author abst.) |
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