The Single-Step Multiplex Reverse Transcription- Polymerase Chain Reaction Assay for Detecting H5 and H7 Avian Influenza A Viruses
|
Accession number;07A0040443
|
| Title;The Single-Step Multiplex Reverse Transcription- Polymerase Chain Reaction Assay for Detecting H5 and H7 Avian Influenza A Viruses |
| Author;
THONTIRAVONG AUNYARATANA
(Faculty of Veterinary Science, Faculty of Medicine, Chulalongkorn University)
PAYUNGPORN SUNCHAI
(Center of Excellence in Viral Hepatitis Research, Faculty of Medicine, Chulalongkorn University)
KEAWCHAROEN JUTHATIP
(Faculty of Veterinary Science, Faculty of Medicine, Chulalongkorn University)
CHUTINIMITKUL SALIN
(Center of Excellence in Viral Hepatitis Research, Faculty of Medicine, Chulalongkorn University)
WATTANODORN SUMITRA
(Faculty of Veterinary Science, Faculty of Medicine, Chulalongkorn University)
DAMRONGWATANAPOKIN SUDARAT
(Department of Livestock Development, National Institute of Animal Health)
CHAISINGH ARUNEE
(Department of Livestock Development, National Institute of Animal Health)
THEAMBOONLERS APIRADEE
(Center of Excellence in Viral Hepatitis Research, Faculty of Medicine, Chulalongkorn University)
POOVORAWAN YONG
(Center of Excellence in Viral Hepatitis Research, Faculty of Medicine, Chulalongkorn University)
ORAVEERAKUL KANISAK
(Faculty of Veterinary Science, Faculty of Medicine, Chulalongkorn University)
|
Journal Title;Tohoku J Exp Med
|
Journal Code:G0649A
|
ISSN:0040-8727
|
|
VOL.211;NO.1;PAGE.75-79 (J-STAGE)(2007)
|
| Figure&Table&Reference;FIG.1, TBL.1, REF.12 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Avian influenza (AI) A virus subtypes H5 and H7 cause severe disease in domestic poultry, including chickens and turkeys. Moreover, H5 and H7 AI A viruses can cross the species barrier from poultry to humans. In the present study, we have developed a single-step multiplex reverse transcription-polymerase chain reaction assay (RT-PCR) for detecting H5 and H7 AI A viruses. This assay was applied to the poultry isolates with the aim of establishing a surveillance method to monitor possible transmission to humans. Two subtype-specific primer sets capable of producing PCR products of 157 and 326 base pairs corresponding to AI A virus H5 and H7 subtypes, respectively, were utilized in a one-step and one-tube reaction. The single-step multiplex RT-PCR assay developed in this study was found to be specific for detecting H5 and H7 AI A viruses. No specific amplification bands were detected with total nucleic acids extracted from other influenza hemagglutinin subtypes and other viral pathogens. The sensitivity of this assay was about 10'3' RNA copies/.MU.l. In conclusion, this novel single-step multiplex RT-PCR is a simple assay with high potential for rapid, specific and cost effective laboratory diagnosis of H5 and H7 AI A virus isolates from clinical specimens of poultry. (Author abst.) |
|
|
|
Related Articles;
|
|
|
