Distinct Effects of Pravastatin, Atorvastatin, and Simvastatin on Insulin Secretion from a .BETA.-cell Line, MIN6 Cells
|
Accession number;07A0040473
|
| Title;Distinct Effects of Pravastatin, Atorvastatin, and Simvastatin on Insulin Secretion from a .BETA.-cell Line, MIN6 Cells |
| Author;
ISHIKAWA MAYUMI
(Dep. of Internal Medicine (Endocrinology and Metabolism) Graduate School of Comprehensive Human ...)
OKAJIMA FUMINORI
(Division of Endocrinology and Metabolism, Department of Medicine, Nippon Medical School.)
INOUE NORIYUKI
(Dep. of Internal Medicine (Endocrinology and Metabolism) Graduate School of Comprehensive Human ...)
MOTOMURA KAORI
(Dep. of Internal Medicine (Endocrinology and Metabolism) Graduate School of Comprehensive Human ...)
KATO TOYONORI
(Dep. of Internal Medicine (Endocrinology and Metabolism) Graduate School of Comprehensive Human ...)
TAKAHASHI AKIMITSU
(Dep. of Internal Medicine (Endocrinology and Metabolism) Graduate School of Comprehensive Human ...)
OIKAWA SHINICHI
(Division of Endocrinology and Metabolism, Department of Medicine, Nippon Medical School.)
YAMADA NOBUHIRO
(Dep. of Internal Medicine (Endocrinology and Metabolism) Graduate School of Comprehensive Human ...)
SHIMANO HITOSHI
(Dep. of Internal Medicine (Endocrinology and Metabolism) Graduate School of Comprehensive Human ...)
SHIMANO HITOSHI
(Center for Tsukuba Advanced Research Alliance, University of Tsukuba.)
|
Journal Title;J Atheroscler Thromb
|
Journal Code:L2187A
|
ISSN:1340-3478
|
|
VOL.13;NO.6;PAGE.329-335 (J-STAGE)(2006)
|
| Figure&Table&Reference;FIG.5, REF.19 |
| Pub. Country;Japan |
| Language;English |
| Abstract;In addition to the prevention of cardiovascular diseases by lowering plasma LDL cholesterol, recent studies suggest that statins could have some impact on insulin action. To estimate the direct effects of statins on insulin secretion from pancreatic .BETA.-cells, MIN6 cells were treated with pravastatin, simvastatin, or atorvastatin. Basal insulin secretion at low glucose concentration was unexpectedly increased at very high doses of simvastatin or atorvastatin after 24- and 48-hour incubation. Insulin secretion at high glucose was not significantly changed, and thus, net glucose-stimulated insulin secretion was apparently decreased by these lipophilic statins. The changes in insulin secretion were highly associated with increased endogenous SREBP activities in response to HMG-CoA inhibition as estimated by SRE-luciferase assays, and finally after 48-hour incubation, accompanied by impaired cell viability as estimated by MTT assays. In contrast, these changes were much less prominent by the addition of pravastatin. Meanwhile, glucose-stimulated insulin secretion of islets isolated from C57BL/6 mice was not significantly changed by any of the statins. Overall, taken up by .BETA.-cells, statins can affect insulin secretion through either HMG-CoA inhibition or cytotoxicity, as observed by the addition of extraordinary high doses of lipophilic statins, but not hydrophilic statins, to the medium. (Author abst.) |
|
|
|
Related Articles;
|
|
|
