Science Links Japan | Association of TNF-.ALPHA. Gene Promoter C-857T Polymorphism with Higher Serum LDL Cholesterol Levels and Carotid Plaque Formation in Japanese Patients with Type 2 Diabetes

Association of TNF-.ALPHA. Gene Promoter C-857T Polymorphism with Higher Serum LDL Cholesterol Levels and Carotid Plaque Formation in Japanese Patients with Type 2 Diabetes

Accession number;07A0229885

Title;Association of TNF-.ALPHA. Gene Promoter C-857T Polymorphism with Higher Serum LDL Cholesterol Levels and Carotid Plaque Formation in Japanese Patients with Type 2 Diabetes

Author; YAMASHINA MITSUHIRO (Department of Diabetes and Metabolism, Iwate Medical University) KANEKO YOSHIHIRO (Department of Diabetes and Metabolism, Iwate Medical University) MAESAWA CHIHAYA (Second Department of Pathology, Iwate Medical University) KAJIWARA TAKASHI (Department of Diabetes and Metabolism, Iwate Medical University) ISHII MOTOTSUGU (Department of Diabetes and Metabolism, Iwate Medical University) FUJIWARA FUMIKADO (Department of Diabetes and Metabolism, Iwate Medical University) TANEICHI HARUHITO (Department of Diabetes and Metabolism, Iwate Medical University) TAKEBE NORIKO (Department of Diabetes and Metabolism, Iwate Medical University) ISHIDA WATARU (Department of Diabetes and Metabolism, Iwate Medical University) TAKAHASHI KAZUMA (Department of Diabetes and Metabolism, Iwate Medical University) MASUDA TOMOYUKI (Second Department of Pathology, Iwate Medical University) SATOH JO (Department of Diabetes and Metabolism, Iwate Medical University)

Journal Title;Tohoku J Exp Med

Journal Code:G0649A

ISSN:0040-8727

VOL.211;NO.3;PAGE.251-258 (J-STAGE)(2007)

Figure&Table&Reference;

Pub. Country;Japan

Language;English

Abstract;Little has been known about the role of tumor necrosis factor-.ALPHA. (TNF-.ALPHA.) gene polymorphisms in metabolic syndrome and atherosclerosis in type 2 diabetes, although TNF-.ALPHA. was reported to be involved in these conditions. We examined the association of TNF-.ALPHA. gene promoter polymorphisms, G-238A, G-308A, C-857T, C-863A, and T-1031C, with metabolic syndrome and surrogate markers of atherosclerosis in Japanese patients with type 2 diabetes. DNA was obtained from 162 patients and TNF-.ALPHA. gene promoter polymorphisms determined by direct sequencing. Allelic frequency of -238A, -308A, -857T, -863A, and -1031C was 0.6%, 2.2%, 11.1%, 16.7%, and 15.7%, respectively. Association of the gene polymorphisms with a number of variables, because of their high frequency, was analyzed in the latter 3 polymorphisms. There were no significant differences in components of metabolic syndrome and variables affecting atherosclerosis, except in case of serum low-density-lipoprotein cholesterol (LDL-C) (111 .+-. 33 vs 125 .+-. 39 mg/dl, p < 0.05) between -857C/C and -857(C/T + T/T). In contrast, no significant differences were found in these markers between -863C/C and -863(C/A + A/A) and between -1031T/T and -1031(T/C + C/C). Furthermore, 87% of the patients with -857(C/T + T/T) and 64% with -857C/C had carotid plaques (p < 0.05). There was no difference in proportion of patients treated with medications such as statins, fibrates, oral hypoglycemic agents, insulin, or antihypertensive drugs between -857C/C and -857(C/T + T/T). These data imply that TNF-.ALPHA. gene polymorphism (C-857T) is likely associated with higher serum LDL-C levels and carotid plaque formation in Japanese patients with type 2 diabetes. (Author abst.)

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