Adenovirus-mediated p53 gene transfer sensitizes hepatocellular carcinoma cells to heavy-ion radiation
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Accession number;07A0256503
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| Title;Adenovirus-mediated p53 gene transfer sensitizes hepatocellular carcinoma cells to heavy-ion radiation |
| Author;
LIU BING
(Inst. Of Modern Physics, Chinese Acad. Of Sci., Gansu Province, Chn)
LIU BING
(Graduate School, Chinese Acad. Of Sci., Beijing, Chn)
ZHANG HONG
(Inst. Of Modern Physics, Chinese Acad. Of Sci., Gansu Province, Chn)
ZHOU GUANGMING
(Inst. Of Modern Physics, Chinese Acad. Of Sci., Gansu Province, Chn)
XIE YI
(Inst. Of Modern Physics, Chinese Acad. Of Sci., Gansu Province, Chn)
XIE YI
(Graduate School, Chinese Acad. Of Sci., Beijing, Chn)
HAO JIFANG
(Inst. Of Modern Physics, Chinese Acad. Of Sci., Gansu Province, Chn)
QIU RONG
(Inst. Of Modern Physics, Chinese Acad. Of Sci., Gansu Province, Chn)
DUAN XIN
(Inst. Of Modern Physics, Chinese Acad. Of Sci., Gansu Province, Chn)
DUAN XIN
(Graduate School, Chinese Acad. Of Sci., Beijing, Chn)
ZHOU QINGMING
(Inst. Of Modern Physics, Chinese Acad. Of Sci., Gansu Province, Chn)
ZHOU QINGMING
(Graduate School, Chinese Acad. Of Sci., Beijing, Chn)
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Journal Title;J Gastroenterol
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Journal Code:Z0748A
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ISSN:0944-1174
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VOL.42;NO.2;PAGE.140-145(2007)
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| Figure&Table&Reference; |
| Pub. Country;Japan |
| Language;English |
| Abstract;Background. The purpose of this study was to investigate whether adenovirus-mediated p53 transfer could sensitize hepatocellular carcinoma to heavy-ion irradiation. Methods. HepG2 cells were preexposed to a '12'C'6+' beam, and then infected with replication-deficient adenovirus recombinant vectors containing human wild-type p53 (AdCMV-p53) ('12'C'6+' irradiation + AdCMV-p53 infection). The survival fraction was determined by clonogenic assay. The cell cycle, cell apoptosis, and p53 expression were monitored by flow cytometric analysis. Results. p53 expression in '12'C'6+' irradiation + AdCMV-p53 infection groups was markedly higher than that in '12'C'6+' irradiation only groups (P<0.05), suggesting that the preexposure to the '12'C'6+' beam promoted the expression of exogenous p53 in HepG2 cells infected with AdCMV-p53 only. The G1-phase arrest and cell apoptosis in the '12'C'6+' irradiation + AdCMV-p53 infection groups were significantly more than those in the '12'C'6+' irradiated groups (P<0.05). The survival fractions of the '12'C'6+' irradiation + AdCMV-p53 infection groups decreased by 30%-49% compared with those of the '12'C'6+' beam-irradiated only groups (P<0.05). Conclusions. Adenovirus-mediated p53 gene transfer can promote G1-phase arrest and cell apoptosis, thus sensitizing hepatocellular carcinoma cells to heavy-ion irradiation. (author abst.) |
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